Next monoclonal antibodies was basically purchased out-of Pharmingen (North park, Calif Cellphone staining and you may move cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaNstep three) with […]
